NR supplementation alleviated malignancy-induced weight loss and metastasis to lung in nude mice...NR supplementation decreased metastasis to the bone and liver...NR supplementation also significantly decreased the size of allografted tumors and extended the survival time... In summary, our results supply evidence that boosting NAD levels by supplementing NR alleviates HCC progression and metastasis, which may serve as an effective treatment for the suppression of liver cancer progression.