NR effects on cells extracted from patients with psoriasis and matched controls ex vivo were assessed to identify gene-metabolic networks mediating NR effects. To further assess these findings, the validation study included a double-blinded clinical trial that recruited healthy participants that took either NR or placebo supplements. Using this approach, and by integrating gene-regulatory and metabolic pathway effects, we have identified an anti-inflammatory role of NR supplementation on Th17 immune responsiveness...NR supplementation blunts Th1 and Th17 immune responsiveness in healthy volunteers and in psoriasis....The finding from this study that NR blunts Th1 and Th17 immune responsiveness in an ex vivo study supports that the utilization of NAD+ boosting could potentially be employed as a supplemental therapy in this disease. The concept is now being explored in vivo, where mild to moderate psoriasis patients are being investigated in a placebo-controlled study...NR supplementation blunts Th1 and Th17 immune responsiveness in healthy volunteers and in psoriasis...This study has expanded our understanding of NAD+ boosting in the adaptive immune system with the focus on NR blunting Th17 immune activity...The finding from this study that NR blunts Th1 and Th17 immune responsiveness in an ex vivo study supports that the utilization of NAD+ boosting could potentially be employed as a supplemental therapy in this disease. The concept is now being explored in vivo, where mild to moderate psoriasis patients are being investigated in a placebo-controlled study...